Difference between revisions of "Part:BBa K1045001"
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This is the ORF of DarR (Ms5346). DarR is a HTH-transcription factor from ''Mycobacterium smegmatis'', which can bind its specific binding sequence (the DarR operator [[Part:BBa_K1045000|BBa_K1045000]]). | This is the ORF of DarR (Ms5346). DarR is a HTH-transcription factor from ''Mycobacterium smegmatis'', which can bind its specific binding sequence (the DarR operator [[Part:BBa_K1045000|BBa_K1045000]]). | ||
| − | In ''M. smegmatis'', it was shown that the binding effinciency of DarR to the operator is enhanced by | + | In ''M. smegmatis'', it was shown that the binding effinciency of DarR to the operator is enhanced by cyclic di-AMP. Upon association with c-di-AMP, DarR binds strongly to the operator shutting down transcription of the downstream gene. This way, transcription is controlled via c-di-AMP (Zhang ''et al''., 2013).<br/ > |
Revision as of 12:49, 19 October 2013
DarR ORF with inversed Pre- and Suffix
This is the ORF of DarR (Ms5346). DarR is a HTH-transcription factor from Mycobacterium smegmatis, which can bind its specific binding sequence (the DarR operator BBa_K1045000).
In M. smegmatis, it was shown that the binding effinciency of DarR to the operator is enhanced by cyclic di-AMP. Upon association with c-di-AMP, DarR binds strongly to the operator shutting down transcription of the downstream gene. This way, transcription is controlled via c-di-AMP (Zhang et al., 2013).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 581
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 594
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 31
- 1000COMPATIBLE WITH RFC[1000]