Difference between revisions of "Part:BBa K1045001"

Revision as of 12:47, 19 October 2013

DarR ORF with inversed Pre- and Suffix

This is the ORF of DarR (Ms5346). DarR is a HTH-transcription factor from Mycobacterium smegmatis, which can bind its specific binding sequence (the DarR operator BBa_K1045000).

In M. smegmatis, it was shown that the binding effinciency of DarR to the operator is enhanced by the availability of cyclic di-AMP. When bound to the operator, DarR shuts down transcription of the gene downstream of its operator. This way, transcription is controlled via c-di-AMP (Zhang et al., 2013).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 581
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 594
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 31
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1045001 short</partinfo>
-This is the ORF of DarR (Ms5346). DarR is a HTH-transcription factor from ''Mycobacterium smegmatis'', which can bind its specific binding sequence (the DarR operator [[Part:BBa_K1045000|BBa_K1045000]]). <br/ >
+This is the ORF of DarR (Ms5346). DarR is a HTH-transcription factor from ''Mycobacterium smegmatis'', which can bind its specific binding sequence (the DarR operator [[Part:BBa_K1045000|BBa_K1045000]]).
 In ''M. smegmatis'', it was shown that the binding effinciency of DarR to the operator is enhanced by the availability of cyclic di-AMP. When bound to the operator, DarR shuts down transcription of the gene downstream of its operator. This way, transcription is controlled via c-di-AMP (Zhang ''et al''., 2013).<br/ >