Difference between revisions of "Part:BBa K1223005"
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<partinfo>BBa_K1223005 short</partinfo> | <partinfo>BBa_K1223005 short</partinfo> | ||
| − | Lambda cI translational unit with | + | Lambda cI translational unit with BBa_K354000 as promoter |
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genebank, iGEM parts registry | genebank, iGEM parts registry | ||
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| + | <!-- Add more about the biology of this part here | ||
| + | ===Usage and Biology=== | ||
===References=== | ===References=== | ||
Revision as of 12:44, 12 October 2013
cI translational unit
Lambda cI translational unit with BBa_K354000 as promoter
Design notes
This part was designed to function as a biological timer for our P.A.S.E 1 system. It includes an assembly of two existing parts from the Registry, Lac/Ara-1 IPTG Inducible promoter (BBa_K354000) and LVA-tagged cI repressor protein (BBa_K327018). In order to extend the half life of the cI protein, we removed the LVA tail. The part was characterized through sequencing and restriction digest with PvuII and HindIII.
lane1: undigested pUC57-cI
lane2: pUC57-cI digested with PvuII and HindIII
lane3: DS5000 ladder
In addition, the promoter's performance under the presence of IPTG and Arabinose was analyzed via comassie staining. The cI protein is 29 KDa.
Source
genebank, iGEM parts registry
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]