Difference between revisions of "Part:BBa K1152010"
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<partinfo>BBa_K1152010 short</partinfo> | <partinfo>BBa_K1152010 short</partinfo> | ||
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− | [[File: | + | [[File:Heidelberg2013_PPtase_svp.png|120px|thumb|'''Figure 1:''' Phenotypes of TOP10 producig indigoidine. As negative control untransformed TOP10 were used.]] |
− | Figure 2 shows the analytical restiction digest we performed for part validation: | + | Svp (Figure 1) encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes (see Figure 2). |
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+ | [[File:Heidelberg2013_PPtase_mechanism.png|280px|thumb|'''Figure 2:''' General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.]] | ||
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+ | Figure 3 shows the analytical restiction digest we performed for part validation: | ||
[[File:Heidelberg2013_BBa_testdigest_CDS.png|280px|thumb|center|'''Figure 2:''' Restriction digest with EcoRI, XbaI, SpeI, PstI.]] | [[File:Heidelberg2013_BBa_testdigest_CDS.png|280px|thumb|center|'''Figure 2:''' Restriction digest with EcoRI, XbaI, SpeI, PstI.]] | ||
Revision as of 18:02, 6 October 2013
Svp 4'-Phosphopanthetheinyl-transferase
Svp (Figure 1) encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes (see Figure 2).
Figure 3 shows the analytical restiction digest we performed for part validation:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 173
Illegal NgoMIV site found at 413
Illegal NgoMIV site found at 663
Illegal AgeI site found at 573 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 366
References
- Takahashi, H., Kumagai, T., Kitani, K., Mori, M., Matoba, Y., & Sugiyama, M. (2007). Cloning and Characterization of a Streptomyces Single Module Type Non-ribosomal Peptide Synthetase Catalyzing a Blue Pigment Synthesis *, 282(12), 9073–9081. doi:10.1074/jbc.M611319200