Difference between revisions of "Part:BBa K823039"

Revision as of 10:09, 6 October 2013 (view source) Jara (Talk | contribs) ← Older edit		Revision as of 12:18, 16 October 2013 (view source) Jara (Talk | contribs) Newer edit →
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	[[File:TUM13 Animation_GFP.gif|thumb|right|320px| Figure: Animated structure of the green fluorescent protein (GFP) based on the cristall structure [http://www.rcsb.org/pdb/explore.do?structureId=2WUR 2WUR]		[[File:TUM13 Animation_GFP.gif|thumb|right|320px| Figure: Animated structure of the green fluorescent protein (GFP) based on the cristall structure [http://www.rcsb.org/pdb/explore.do?structureId=2WUR 2WUR]
	with an identitiy of 98% with this BioBrick. Added by [http://2013.igem.org/Team:TU-Munich TUM 2013 Team]. ]]		with an identitiy of 98% with this BioBrick. Added by [http://2013.igem.org/Team:TU-Munich TUM 2013 Team]. ]]
−	This gfpmut1 gene was amplified from a ''B. subtilis'' vector.	+	This gfpmut1 gene was amplified from the ''B. subtilis'' vector pGFPamy.
−	''Which vector it was, has to be checked again...''	+	Big thanks to the TU-Munich TUM 2013 Team for the advice that this actually is gfpmut1 (not gfpmut3).
	It is constructed in the [[Help:Assembly_standard_25|Freiburg standard]] with RBS.		It is constructed in the [[Help:Assembly_standard_25|Freiburg standard]] with RBS.

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Revision as of 12:18, 16 October 2013

gfpmut1 (B. subtilis optimized + Freiburg standard)

This gfpmut1 gene was amplified from the B. subtilis vector pGFPamy.

Big thanks to the TU-Munich TUM 2013 Team for the advice that this actually is gfpmut1 (not gfpmut3).

It is constructed in the Freiburg standard with RBS.

Find out more about the design of our prefix with ribosome binding site.

prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT

We took a gfp derivate of the Bacillus subtilis plasmids pGFPamy and added the BioBrick compatiple pre- and suffix of the Freiburg standard (Assembly 25). This BioBrick was used to create our [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins Sporobeads]. It is part of the BioBrick K823049

Find out more about our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks Bacillus BioBrick Box] and the created [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins Sporobeads].

Sequence and Features