Difference between revisions of "Part:BBa K1140006:Design"
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===Source=== | ===Source=== | ||
− | Obtained synthetically. The RNA thermosensor sequence was obtained and adapted from [ | + | Obtained synthetically. The RNA thermosensor sequence was obtained and adapted from [4] (u6 synthetic RNA thermometer). The mCherry coding sequence was optimized for E. coli codon usage. |
This composite part use the TetR sequence designed by June Rhee, Connie Tao, Ty Thomson and Louis Waldman (BBa_R0040) in 2003. | This composite part use the TetR sequence designed by June Rhee, Connie Tao, Ty Thomson and Louis Waldman (BBa_R0040) in 2003. |
Revision as of 22:05, 5 October 2013
pTet + 37 oC RNA thermometer + mCherry (LVA)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 63
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 676
Illegal AgeI site found at 788 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.
Source
Obtained synthetically. The RNA thermosensor sequence was obtained and adapted from [4] (u6 synthetic RNA thermometer). The mCherry coding sequence was optimized for E. coli codon usage.
This composite part use the TetR sequence designed by June Rhee, Connie Tao, Ty Thomson and Louis Waldman (BBa_R0040) in 2003.
References
1. Elowitz MB, and Leibler S, (2000). A synthetic oscillatory network of transcriptional regulators. Nature, 20;403(6767):335-8.
2. Duval-Valentin G, and Ehrlich R, (1986). Interaction between E. coli RNA polymerase and the tetR promoter from pSC101: homologies and differences with other E. coli promoter systems from close contact point studies. Nucleic Acids Res., 14(5):1967-83.
3. Neupert J, Karcher D, Bock R, (2008). Design of simple synthetic RNA thermometers for temperature- controlled gene expression in Escherichia coli. Nucleic Acids Res, 36(19):e124.