Difference between revisions of "Part:BBa K1216002:Design"
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− | We obtain the plasmid with the hydrolase from Johannes Haerle of the Panke Group from D-BSSE ETHZ in Basel | + | We obtain the plasmid with the hydrolase from Johannes Haerle of the Panke Group from D-BSSE ETHZ in Basel, checked them for biobrick restriction sites and sequenced them to confirm the sequence. We did PCR with overlapping fragments to introduce the biobrick pre and suffix to clone them into biobrick vectors. |
===Source=== | ===Source=== |
Revision as of 03:51, 5 October 2013
Acetyl esterase (aes) from Escherichia Coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 27
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 951
Design Notes
NO design considerations.
We obtain the plasmid with the hydrolase from Johannes Haerle of the Panke Group from D-BSSE ETHZ in Basel, checked them for biobrick restriction sites and sequenced them to confirm the sequence. We did PCR with overlapping fragments to introduce the biobrick pre and suffix to clone them into biobrick vectors.
Source
Escherichia coli