Difference between revisions of "Part:BBa K1197012"

Revision as of 03:36, 5 October 2013

Pveg + RBS + Sam 8 + DT

P-coumaric acid production: The aim of modeling P-coumaric Acid production is to predict the amount and the activity of this enzyme ,Sam8, and the production of p-coumaric acid. The model was created mainly by designing ODEs and enzyme kinetics equations .Then; these equations were solved by the help of MATLAB software.

In our circuit, Sam8 gene is transcribed with B. subtilis specific constituve promoter Pveg. SacB is transcribed with Sam8 gene for secretion out of cell.

• We have calculated our enzyme activity according to our enzyme production rate. In 3500 s , 12E9 molecule of p-coumaric acid is produced according to our model.

Sam8 activity:

References:

1- https://static.igem.org/mediawiki/2009/6/6c/ODEs.pdf

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1053
Illegal BamHI site found at 1455
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 796
Illegal AgeI site found at 743
Illegal AgeI site found at 912
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 347
Illegal BsaI site found at 613

@@ Line 7: / Line 7: @@
 In our circuit, Sam8 gene is transcribed with B. subtilis specific constituve promoter Pveg.  SacB is transcribed with Sam8 gene for secretion out of cell.
-Mathematical model:
-Differential equations:
-• In our model, we calculated the
+• We have calculated our enzyme activity according to our enzyme production rate. In 3500 s ,  12E9 molecule of p-coumaric acid is produced according to our model.
-Simulation:
-• We have calculated our enzyme activity according to our enzyme production rate. In 3500 s ,  12E9 molecule of p-coumaric acid is produced according to our model.
+'''Sam8 activity:'''
+https://static.igem.org/mediawiki/parts/2/2d/P-coumaric_acid.jpg
 References: