Difference between revisions of "Part:BBa K1197012"

Revision as of 03:17, 5 October 2013

Pveg + RBS + Sam 8 + DT

P-coumaric acid production: The aim of modeling P-coumaric Acid production is to predict the amount and the activity of this enzyme ,Sam8, and the production of p-coumaric acid. The model was created mainly by designing ODEs and enzyme kinetics equations .Then; these equations were solved by the help of MATLAB software.

In our circuit, Sam8 gene is transcribed with B. subtilis specific constituve promoter Pveg. SacB is transcribed with Sam8 gene for secretion out of cell.

Mathematical model: Differential equations:

• In our model, we calculated the

Simulation:

• We have calculated our enzyme activity according to our enzyme production rate. In 3500 s , 12E9 molecule of p-coumaric acid is produced according to our model. References:

1- https://static.igem.org/mediawiki/2009/6/6c/ODEs.pdf

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Unknown
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
INCOMPATIBLE WITH RFC[23]
Unknown
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 796
Illegal AgeI site found at 743
Illegal AgeI site found at 912
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 347
Illegal BsaI site found at 613

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1197012 short</partinfo>
- P- coumaric Acid Production:
+P-coumaric acid production:
 The aim of modeling P-coumaric Acid production is to predict the amount and the activity of this enzyme ,Sam8, and the production of  p-coumaric acid. The model was created mainly by designing ODEs and enzyme kinetics equations .Then; these equations were solved by the help of MATLAB software.