Difference between revisions of "Part:BBa K1150043"

Revision as of 23:31, 4 October 2013

uniCAS RNAimer multiple targets to VEGF 2 and VEGF 3

pH1:tracrRNA pU6:DR_dummy_DR
Function	two small RNAs giving rise to specific binding of dCas9 to two VEGF loci
Use in	Mammalian cells
RFC standard	RFC 25
Backbone	pSB1C3
Organism	Streptococcus pyogenes
Source	Feng Zhang, Addgene
Submitted by	[http://2013.igem.org/Team:Freiburg Freiburg 2013]

Usage and Biology

By combining two single loci via the idempotent cloning strategy two loci were connected into one RNA plasmid. This enables multiple targeting of two loci with the [http://2013.igem.org/Team:Freiburg/Project/effector dCas9 derivates] at once. The basis of the device is the RNAimer.
Here, we combined BBa_K1150037 and BBa_K1150038.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 224
Illegal BglII site found at 1061
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 216
Illegal BsaI.rc site found at 1053

@@ Line 33: / Line 33: @@
 ===Usage and Biology===
-By combining two single loci via the idempotent cloning strategy two loci were connected into one RNA plasmid. This enables multiple targeting of two loci with the [http://2013.igem.org/Team:Freiburg/Project/effector dCas9 derivates] at once. The basis of the device is the [https://parts.igem.org/Part:BBa_K1150034 RNAimer].
+By combining two single loci via the idempotent cloning strategy two loci were connected into one RNA plasmid. This enables multiple targeting of two loci with the [http://2013.igem.org/Team:Freiburg/Project/effector dCas9 derivates] at once. The basis of the device is the [https://parts.igem.org/Part:BBa_K1150034 RNAimer].<br>
 Here, we combined [https://parts.igem.org/Part:BBa_K1150037 BBa_K1150037] and [https://parts.igem.org/Part:BBa_K1150038 BBa_K1150038].