Difference between revisions of "Part:BBa K1150040"
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Freiburg 2013 used this plasmid to test the efficiency of target Bla2 by activating or repressing a SEAP reporter gene. | Freiburg 2013 used this plasmid to test the efficiency of target Bla2 by activating or repressing a SEAP reporter gene. | ||
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| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'> |
| + | ==Sequence and Features== | ||
| + | </span> | ||
<partinfo>BBa_K1150040 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1150040 SequenceAndFeatures</partinfo> | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 02:31, 5 October 2013
uniCAS RNAimer to Bla Target 2
| RNaimer (Bla target 2) | |
|---|---|
| Function | Targeting VEGF2 with Cas9 |
| Use in | Mammalian cells |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered dCas9 protein to the Bla locus target 2 . This RNaimer is bulid of the uniCAS RNAimer and contains the DNA sequence coding for a crRNA that binds complementary to Bla 2 target.
Usage and Biology
Freiburg 2013 used this plasmid to test the efficiency of target Bla2 by activating or repressing a SEAP reporter gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 224
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 216