Difference between revisions of "Part:BBa K1150041"

(Usage and Biology)
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|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
 
|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
 
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This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered [https://parts.igem.org/Part:BBa_K1150000 dCas9] protein to the Bla locus target 3 .  This RNaimer is bulid of the  [https://parts.igem.org/Part:BBa_K1150034 uniCAS RNAimer] and contains the DNA sequence coding for a crRNA that binds complementary to Bla 3 target.  
 
This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered [https://parts.igem.org/Part:BBa_K1150000 dCas9] protein to the Bla locus target 3 .  This RNaimer is bulid of the  [https://parts.igem.org/Part:BBa_K1150034 uniCAS RNAimer] and contains the DNA sequence coding for a crRNA that binds complementary to Bla 3 target.  

Revision as of 23:20, 4 October 2013

uniCAS RNaimer to Bla Target 3


RNaimer (Bla target 3)
Function Targeting VEGF3 with Cas9
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered dCas9 protein to the Bla locus target 3 . This RNaimer is bulid of the uniCAS RNAimer and contains the DNA sequence coding for a crRNA that binds complementary to Bla 3 target.

Usage and Biology

Freiburg 2013 used this plasmid to test the efficiency of target Bla3 by activating or repressing a SEAP reporter gene.