Difference between revisions of "Part:BBa K1155006:Experience"
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Revision as of 22:08, 4 October 2013
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Applications of BBa_K1155006
We have tested the activity of BBa_K1155006 by observing the expression of lacZ.
Construction: BBa_K1155006 + BBa_K1155007 in pSB1K3 ( construction obtained not yet sequenced)
Strains were plated on LB + Xgal and incubated in anaerobic conditions.
What we expected :
+ O2 : FNR inactivated : PnarK repressed --> no color
- O2 : FNR activated : PnarK activated --> blue color
What we observed :
+ O2 : --> not tested
- O2 : --> blue color
[photo]
These results indicate that the PnarK(BBa_K1155004) seems to be well induced by FNR in anaerobic condition. The repression of these construction has not been tested in aerobic conditions.
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