This biobrick did measure up to our expectations as shown in the following data. We also used this promoter to express our PET cleaving enzyme pNB-Est13, which is anchored C-terminal to EstA.(''E. Coli'' membrane anchor protein). AraC-Pbad shows a low background activity and a good respose to the induction with Arabinose.
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[[Image:Spectra_LssmOrange.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
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[[Image:MKate-LSSmOrange_FRET_Pair.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
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[[Image:LssmOrange_bac.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
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[[Image:Grow_log.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
Revision as of 02:25, 5 October 2013
LssmOrange is a fluorophor derived from dsRed
LssmOrange is a fluorophor derived from dsRed. It features the excitation maxima at 437 nm and the emission maxima at 571 nm.
