Difference between revisions of "Part:BBa K1152001"

Latest revision as of 21:06, 4 October 2013

TycB1 Module from Tyrocidine

This is the coding sequence for the TycB1 module from a gene cluster of Brevibacillus parabrevis. TycB1 is the second module in the cluster encoding the non-ribosomal peptide (NRP) synthetase of the cyclic decamer Tyrocidine. This specific module TycB1 is responsiple for the binding of the second amino acid Proline of the NRP. It consists of a C- (condensation), an A- (adenylation) and a PCP- (peptide-carrier-protein) domain. After Tyc B1 follows the TycB2 module of the tycB gene, which has three modules.

This biobrick can be used for the design of different NRPS. It was used in the synthetic Dipeptide and TetrapeptideI/II of our [http://2013.igem.org/Team:Heidelberg/Project/Tyrocidine project].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1195

@@ Line 5: / Line 5: @@
 TycB1 is the second module in the cluster encoding the non-ribosomal peptide (NRP) synthetase of the cyclic decamer Tyrocidine.
 This specific module TycB1 is responsiple for the binding of the second amino acid Proline of the NRP.
-It consists of the C- (condensation), A- (adenylation) and the PCP- (peptide-carrier-protein) domain. After Tyc B1 follows the TycB2 module of the tycB gene, which has three modules.
+It consists of a C- (condensation), an A- (adenylation) and a PCP- (peptide-carrier-protein) domain. After Tyc B1 follows the TycB2 module of the tycB gene, which has three modules.
 This biobrick can be used for the design of different NRPS. It was used in the synthetic Dipeptide and TetrapeptideI/II of our [http://2013.igem.org/Team:Heidelberg/Project/Tyrocidine project].