Difference between revisions of "Part:BBa K1104204"
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<partinfo>BBa_K1104204 short</partinfo> | <partinfo>BBa_K1104204 short</partinfo> | ||
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+ | AhpCp2D1 is a ROS-induced promoter, which is controlled by OxyR (transcription factor) activated by ROS (Reactive Oxygen Species). | ||
+ | [[File: NYMU_A2D1x.png|thumb|600px|center|'''Mutation of ahpC promoter([https://parts.igem.org/Part:BBa_K362001 Part:K362001])''']] | ||
AhpCp2D1 is composed of AhpCp2 ([https://parts.igem.org/Part:BBa_K1104205 Part:BBa_K1104205]), reverse promoter DsbGp ([https://parts.igem.org/Part:BBa_K1104208 Part:BBa_K1104208]),and AhpCp1 ([https://parts.igem.org/Part:BBa_K1104207 Part:BBa_K1104207]). There are also two dual-TFBSs (Transcription Factor Binding Site) for OxyR binding between AhpCp2 ([http://ptruncated coding sequence from the promoter sequencetruncated coding sequence from the promoter sequencetruncated coding sequence from the promoter sequencebG coding)from the promoter sequence. | AhpCp2D1 is composed of AhpCp2 ([https://parts.igem.org/Part:BBa_K1104205 Part:BBa_K1104205]), reverse promoter DsbGp ([https://parts.igem.org/Part:BBa_K1104208 Part:BBa_K1104208]),and AhpCp1 ([https://parts.igem.org/Part:BBa_K1104207 Part:BBa_K1104207]). There are also two dual-TFBSs (Transcription Factor Binding Site) for OxyR binding between AhpCp2 ([http://ptruncated coding sequence from the promoter sequencetruncated coding sequence from the promoter sequencetruncated coding sequence from the promoter sequencebG coding)from the promoter sequence. | ||
Revision as of 20:35, 4 October 2013
AhpCp2D1
AhpCp2D1 is a ROS-induced promoter, which is controlled by OxyR (transcription factor) activated by ROS (Reactive Oxygen Species).
AhpCp2D1 is composed of AhpCp2 (Part:BBa_K1104205), reverse promoter DsbGp (Part:BBa_K1104208),and AhpCp1 (Part:BBa_K1104207). There are also two dual-TFBSs (Transcription Factor Binding Site) for OxyR binding between AhpCp2 ([http://ptruncated coding sequence from the promoter sequencetruncated coding sequence from the promoter sequencetruncated coding sequence from the promoter sequencebG coding)from the promoter sequence.
How ahpCp2D1 (Part:BBa_K362001) is improved?
We annotated it thouroughly based on data from ([http://ecocyc.org/ECOLI/new-image?object=EG11384 Ecocyc Ecocyc]), and found that it contains dsbG coding sequence, AhpCp2 (Part:BBa_K1104205), reverse promoter DsbGp (Part:BBa_K1104208),and AhpCp1 (Part:BBa_K1104207), and a PstI cutting site. Thus we improved the promoter by first mutating the PstI cutting site in ahpCp (Part:BBa_K362001) and make dsbG coding removed.
Here is the overview about the other ahpC promoter (Part:BBa_K362001) improvements:
- AhpCp1000 (Part:BBa_K1104204): The PstI cutting site is mutated.
- AhpCp2 (Part:BBa_K1104205): Only one promoter(AhpCp2) and its TFBS.
- AhpCpD1 (Part:BBa_K1104206): Bidirectional promoter: AhpCp1 and DsbGp(reverse promoter), and their shared TFBS.
- AhpCp1 (Part:BBa_K1104207): Only one promoter(AhpCp1) and its TFBS.
- DsbGp (Part:BBa_K1104208): Only the reverse promoter(DsbGp) and its TFBS.
Usage and Biology
We designed circuit fighting against Nosema ceranae. After Nosema ceranae infected midgut cells of bees, and Bee. coli should sense the pathogen first before the following circuit(fighting against Nosema ceranae)is triggered, and substance such as Defensin(Part:BBa_K1104300), Abaesin(Part:BBa_K1104301) (more details on [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Killing Killing Nosema] page) in the following circuit will express.
To enhance the strength , we added a device (more details on [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema] page).
Related Parts
- ahpC(Part:BBa_K362001)
- AhpCp1000(Part:BBa_K1104203)
- Part:BBa_K1104243: AhpCp1000+E0840
- AhpCp2D1(Part:BBa_K1104204)
- Part:BBa_K1104244: AhpCp2D1+E0840
- AhpCp2(Part:BBa_K1104205)
- Part:BBa_K1104245: AhpCp2+E0840
- AhpCpD1(Part:BBa_K1104206)
- Part:BBa_K1104246: AhpCpD1+E0840
- AhpCp1(Part:BBa_K1104207)
- Part:BBa_K1104247: AhpCp1+E0840
- DsbGp(Part:BBa_K1104208)
- Part:BBa_K1104248: DsbGp+E0840
- AhpCp1000(Part:BBa_K1104203)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]