Difference between revisions of "Part:BBa K1045014:Design"
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===Source=== | ===Source=== | ||
| − | The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence of the promoter originated from the Parts Registry page of [[Part:BBa_J23117|BBa_J23117]]. The 54 bp upstream of the promoter represent a random sequence which would translate into "cyclicdiampacterim". | + | The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence of the promoter originated from the Parts Registry page of [[Part:BBa_J23117|BBa_J23117]]. The 54 bp upstream of the promoter represent a random sequence which would translate into "cyclicdiampacterim". For the origin of [[Part:BBa_J23117|BBa_J23117]], see [[https://parts.igem.org/Part:BBa_K1045013:Design]]. |
===References=== | ===References=== | ||
Revision as of 19:48, 4 October 2013
Promoter reverse - Promoter - DarR operator - GFP generator BBa_E0240
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
Illegal NheI site found at 24
Illegal NheI site found at 104
Illegal NheI site found at 127 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 55
Illegal AgeI site found at 963 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 825
Design Notes
This part was constructed using hybridization oligos for BBa_K1045011. The hybridization product corresponded to a DNA fragment harboring the sequence of BBa_K1045011 cut with EcoRI and SpeI at the prefix and suffix sites. This fragment was ligated into BBa_K1045013 in a prefixing composition.
This biobrick harbors a mutation in the suffix sequence. The mutated suffix sequence is: TACTAGTAACGGCCGCTGCAG. This eliminates the NotI restriction site. The plasmid might still be cut with SpeI and PstI.
Source
The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence of the promoter originated from the Parts Registry page of BBa_J23117. The 54 bp upstream of the promoter represent a random sequence which would translate into "cyclicdiampacterim". For the origin of BBa_J23117, see [[1]].