Difference between revisions of "Part:BBa K1033112"

Revision as of 19:16, 4 October 2013

Uracil glucosyltransferase (UGTCs2)

It is believed that Uracil glucosyltransferase from Crocus Sativus (UGTCs2) cleaves products catalyzed by the enzyme zeaxanthin 7,8-cleavage dioxygenase (ZCD) and that it is neccesary for the production of crocin glucosides, safranal and picrocrocin. UGTCs2 was obtained from WashU 2012 in a synthetic construct togeher with ZCD. The genes were isolated by the use of primers with overhangs. The overhangs contained a RBS (B0034) but overhangs without RBS were also used for isolation for future uses. We assembled with promoters but without success but when used mutagenesis we managed to attach several promoters of the J23-series. They were sequenced and tested in Western Blot, the sequences were correct but unfortunately we could not see a protein expression. This would show that we failed to make the Western Blot but the Sequencing shows that the promoter was correctly assembled.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1033112 short</partinfo>
-It is believed that Uracil glucosyltransferase from Crocus Sativus (UGTCs2) cleaves products catalyzed by the enzyme zeaxanthin 7,8-cleavage dioxygenase (ZCD) and that it is neccesary for the production of crocin glucosides, safranal and picrocrocin. UGTCs2 was obtained from WashU 2012 in a synthetic construct togeher with ZCD. The genes were isolated by the use of primers with overhangs. The overhangs contained a RBS (B0034) but overhangs without RBS were also used for isolation for future uses. We assembled with promoters but without success but when used mutagenesis we managed to attach several promoters of the J23-series. They were sequenced and tested in Western Blot, the sequences were correct but unfortunately we could not see a protein expression. This would show that we failed to make the Western Blot.
+It is believed that Uracil glucosyltransferase from Crocus Sativus (UGTCs2) cleaves products catalyzed by the enzyme zeaxanthin 7,8-cleavage dioxygenase (ZCD) and that it is neccesary for the production of crocin glucosides, safranal and picrocrocin. UGTCs2 was obtained from WashU 2012 in a synthetic construct togeher with ZCD. The genes were isolated by the use of primers with overhangs. The overhangs contained a RBS (B0034) but overhangs without RBS were also used for isolation for future uses. We assembled with promoters but without success but when used mutagenesis we managed to attach several promoters of the J23-series. They were sequenced and tested in Western Blot, the sequences were correct but unfortunately we could not see a protein expression. This would show that we failed to make the Western Blot but the Sequencing shows that the promoter was correctly assembled.
 <!-- Add more about the biology of this part here