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===Usage and Biology===
 
===Usage and Biology===
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Revision as of 19:00, 4 October 2013

Miraculin

None, this construct contains no RBS or promoter

Results

We were successful in both subcloning the synthetic construct to a desired plasmid and isolating the Maraculin gene by itself. The sequencencing was done at GATC biotch and Uppsala Genome center. We were not successful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]