Difference between revisions of "Part:BBa K1045010:Experience"

Revision as of 18:57, 4 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1045010

We used this part in our DarR reporter system BBa_K1045017. BBa_K1045010 seemed to have been functional as our characterization experiments of BBa_K1045017 suggested. In this system, gfp is placed under the control of the DarR operator BBa_K1045000. We saw that GFP expression was repressed when E. coli harbored a construct carrying a DarR expression unit with BBa_K1045010 as an RBS. For more information, see main page of BBa_K1045017 and our entry on the experience page of BBa_K1045017: [1].

User Reviews

UNIQe18a3345a7ea7de7-partinfo-00000000-QINU UNIQe18a3345a7ea7de7-partinfo-00000001-QINU

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 ===Applications of BBa_K1045010===
-We used this part in our DarR reporter system [[Part:BBa_K1045017|BBa_K1045017]]. '''BBa_K1045010''' seemed to have been functional as our characterization experiments of [[Part:BBa_K1045017|BBa_K1045017]] suggested. We saw that GFP expression was repressed when ''E. coli'' harbored a construct carrying a DarR expression unit with '''BBa_K1045010''' as an RBS. For more information, see main page of [[Part:BBa_K1045017|BBa_K1045017]] and our entry on the experience page of BBa_K1045017: [https://parts.igem.org/Part:BBa_K1045017:Experience].
+We used this part in our DarR reporter system [[Part:BBa_K1045017|BBa_K1045017]]. '''BBa_K1045010''' seemed to have been functional as our characterization experiments of [[Part:BBa_K1045017|BBa_K1045017]] suggested. In this system, ''gfp'' is placed under the control of the DarR operator [[Part:BBa_K1045000|BBa_K1045000]]. We saw that GFP expression was repressed when ''E. coli'' harbored a construct carrying a DarR expression unit with '''BBa_K1045010''' as an RBS. For more information, see main page of [[Part:BBa_K1045017|BBa_K1045017]] and our entry on the experience page of BBa_K1045017: [https://parts.igem.org/Part:BBa_K1045017:Experience].
 ===User Reviews===