Difference between revisions of "Part:BBa K1045006:Design"

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===References===
 
===References===
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Peter Y Watson & Martha J Fedor (2012) “The ''ydaO'' motif is an c-di-AMP-sensing riboswitch in ''Bacillus subtilis''“, Nature Chemical Biology No. 8, pp. 963-965
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Peter Y Watson & Martha J Fedor (2012) “The ''ydaO'' motif is an ATP-sensing riboswitch in ''Bacillus subtilis''“, Nature Chemical Biology No. 8, pp. 963-965

Revision as of 19:28, 4 October 2013

YdaO Riboswitch with native Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into EcoRI and PstI digested pSB1C3 backbone.

Source

This part was generated by PCR amplification of B. subtilis chromosomal DNA.

References

Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965