Difference between revisions of "Part:BBa K1045000:Design"

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===References===
 
===References===
  
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Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096
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Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in ''Mycobacterium smegmatis''”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096

Revision as of 19:19, 4 October 2013

DarR operator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Vector.jpg

Source

The sequence of this part corresponds to the sequence of the DarR operator upstream of darR, which was published by Zhang et al. in 2013. The part was generated using hybridization oligos purchased from Sigma-Aldrich.

[http://www.google.de/imgres?um=1&hl=de&biw=1433&bih=691&tbm=isch&tbnid=-_tTmC4jAFeHFM:&imgrefurl=http://2007.igem.org/wiki/index.php/Paris/transclusion_example&docid=4kRb6plD_PfdGM&imgurl=https://static.igem.org/mediawiki/2007/d/d2/Pbluescript_SK-.jpg&w=316&h=241&ei=x8ZCUsOgPMrg4QSExIGoCQ&zoom=1&iact=hc&vpx=427&vpy=229&dur=1608&hovh=192&hovw=252&tx=52&ty=213&page=1&tbnh=161&tbnw=199&start=0&ndsp=34&ved=1t:429,r:3,s:0,i:89 Vector iGEM 2007 Wiki]

References

Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096