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[[File:Freiburg2013 Plasmid Cas9-KRAB-1.jpg|800px|thumb|center|<b>Fig. 1</b> Schematic overview of dCas9-KRAB composite part with all features.]]  
  
  

Revision as of 17:42, 4 October 2013

uniCAS Repressor (SV40 promoter)

SV40:HA-NLS-dCas9-Linker-KRAB-NLS:BGH
Function Transcriptional Repression
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Organism Streptococcus pyogenes, Homo sapiens
Source Feng Zhang, Addgene
Konrad Müller, University of Freiburg
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]
Fig. 1 Schematic overview of dCas9-KRAB composite part with all features.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4784
    Illegal SapI.rc site found at 4748