Difference between revisions of "Part:BBa K1150022"
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'''Figure 1:''' Schematic overview of dCas9-KRAB composite part with all features. | '''Figure 1:''' Schematic overview of dCas9-KRAB composite part with all features. | ||
Revision as of 17:37, 4 October 2013
uniCAS Repressor (CMV promoter)
| CMV:HA-NLS-dCas9-Linker-KRAB-NLS:BGH | |
|---|---|
| Function | Transcriptional Repression |
| Use in | Mammalian cells |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Organism | Streptococcus pyogenes, Homo sapiens |
| Source | Feng Zhang, Addgene Konrad Müller, University of Freiburg |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
File:Https://static.igem.org/mediawiki/parts/a/a8/Freiburg2013 Plasmid Cas9-KRAB-1.jpg
Figure 1: Schematic overview of dCas9-KRAB composite part with all features.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
Illegal BglII site found at 900 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 5020
Illegal SapI.rc site found at 4984