Difference between revisions of "Part:BBa K1150022"
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|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
 
|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
 
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[[File:Fhttps://static.igem.org/mediawiki/parts/a/a8/Freiburg2013_Plasmid_Cas9-KRAB-1.jpg|800px|]] <br>
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'''Figure 1:''' Schematic overview of dCas9-KRAB composite part with all features.
  
 
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Revision as of 17:37, 4 October 2013

uniCAS Repressor (CMV promoter)

CMV:HA-NLS-dCas9-Linker-KRAB-NLS:BGH
Function Transcriptional Repression
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Organism Streptococcus pyogenes, Homo sapiens
Source Feng Zhang, Addgene
Konrad Müller, University of Freiburg
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

File:Fhttps://static.igem.org/mediawiki/parts/a/a8/Freiburg2013 Plasmid Cas9-KRAB-1.jpg
Figure 1: Schematic overview of dCas9-KRAB composite part with all features.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 900
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5020
    Illegal SapI.rc site found at 4984