Difference between revisions of "Part:BBa K1060009"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1060009 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1060009 SequenceAndFeatures</partinfo> | ||
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+ | ==Construct digestion== | ||
+ | |||
https://static.igem.org/mediawiki/2013/a/a1/BBa_K1060009_gel_pic.jpg | https://static.igem.org/mediawiki/2013/a/a1/BBa_K1060009_gel_pic.jpg | ||
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− | http://www.youtube.com/watch?v=n-pMp8-XdUo | + | |
+ | ==System testing== | ||
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+ | [http://www.youtube.com/watch?v=n-pMp8-XdUo Aphid Repellence video] | ||
+ | |||
+ | For more infromation see experience or ADD LINK. |
Revision as of 17:37, 4 October 2013
Medium constitutive expression of EBF synthase from Artemisia annua
For the production of the alarmhormone,(E)-β-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a medium (constitutive) promoter and medium RBS (BBa_K608006).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 177 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1368
Illegal BamHI site found at 677
Illegal XhoI site found at 639 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 249
Construct digestion
System testing
[http://www.youtube.com/watch?v=n-pMp8-XdUo Aphid Repellence video]
For more infromation see experience or ADD LINK.