Difference between revisions of "Part:BBa K1104100"
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mngB, a passage of gene in ''Escherichia coli'' K-12 substrain MG1655, can produce alpha-mannosidase, which is involved in the cleavage of the alpha form of mannose. The alpha-mannosidase reaction is to turn 2-O-(6-phospho-α-mannosyl)-D-glycerate into mannose-6-phosphate and glycerate. | mngB, a passage of gene in ''Escherichia coli'' K-12 substrain MG1655, can produce alpha-mannosidase, which is involved in the cleavage of the alpha form of mannose. The alpha-mannosidase reaction is to turn 2-O-(6-phospho-α-mannosyl)-D-glycerate into mannose-6-phosphate and glycerate. | ||
| − | + | ==Usage and Biology== | |
| − | + | ||
The mannose degradation pathway is often seen in many organisms, such as the reverse reaction of the post-translation (glycosylation) on polar filaments of Fungi. The reason why we choose mannosidase gene from MG1655 is that this species of ''E-coli'' are able to live in bees' midgut. That way, this enzyme can well perform in our ''Bee. coli''. We tend to overexpress this gene in order to inhibit the production of the polar protein in the sprouting of ''Nosema. ceranae'', the target fungi that we are eager to eliminate. | The mannose degradation pathway is often seen in many organisms, such as the reverse reaction of the post-translation (glycosylation) on polar filaments of Fungi. The reason why we choose mannosidase gene from MG1655 is that this species of ''E-coli'' are able to live in bees' midgut. That way, this enzyme can well perform in our ''Bee. coli''. We tend to overexpress this gene in order to inhibit the production of the polar protein in the sprouting of ''Nosema. ceranae'', the target fungi that we are eager to eliminate. | ||
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Latest revision as of 13:27, 4 October 2013
mngB - alpha-mannosidase
Introduction
mngB, a passage of gene in Escherichia coli K-12 substrain MG1655, can produce alpha-mannosidase, which is involved in the cleavage of the alpha form of mannose. The alpha-mannosidase reaction is to turn 2-O-(6-phospho-α-mannosyl)-D-glycerate into mannose-6-phosphate and glycerate.
Usage and Biology
The mannose degradation pathway is often seen in many organisms, such as the reverse reaction of the post-translation (glycosylation) on polar filaments of Fungi. The reason why we choose mannosidase gene from MG1655 is that this species of E-coli are able to live in bees' midgut. That way, this enzyme can well perform in our Bee. coli. We tend to overexpress this gene in order to inhibit the production of the polar protein in the sprouting of Nosema. ceranae, the target fungi that we are eager to eliminate. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 716
Illegal BglII site found at 992
Illegal BglII site found at 2139 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 235
Illegal AgeI site found at 2242 - 1000COMPATIBLE WITH RFC[1000]