Difference between revisions of "PBAD SPL"

(DTU pBAD SPL)
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Here the DTU iGEM team will present their work on characterizing a SPL of pBAD promoters.
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This is a page containing a synthetic promoter library (SPL) of pBAD promoters made by [http://2013.igem.org/Team:DTU-Denmark 2013 DTU iGEM team]. The library can be used to make and arabinose inducible system with very low leakiness, or tune the the promoter strength of the system when it's turned on.
  
List of sequences:
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A tight inducible system can be a very useful tool to express lethal or growth inhibiting proteins in ''E.coli''. It can also be necessary with a non leaky expression system if you would like to visualize the expression of GFP SF in ''E.coli'' (see [http://2013.igem.org/Team:DTU-Denmark/HelloWorld link]). 
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Here is a complete list of the sequences and their relative leakiness and strength:
  
==DTU pBAD SPL==
 
 
{|class="sortable" style="border:3px solid black; width: 70%" align="center"  cellspacing="0"
 
{|class="sortable" style="border:3px solid black; width: 70%" align="center"  cellspacing="0"
 
! style="background:#AAAAAA; color:black"  |Identifier
 
! style="background:#AAAAAA; color:black"  |Identifier

Revision as of 16:56, 4 October 2013

This is a page containing a synthetic promoter library (SPL) of pBAD promoters made by [http://2013.igem.org/Team:DTU-Denmark 2013 DTU iGEM team]. The library can be used to make and arabinose inducible system with very low leakiness, or tune the the promoter strength of the system when it's turned on.

A tight inducible system can be a very useful tool to express lethal or growth inhibiting proteins in E.coli. It can also be necessary with a non leaky expression system if you would like to visualize the expression of GFP SF in E.coli (see [http://2013.igem.org/Team:DTU-Denmark/HelloWorld link]).


Here is a complete list of the sequences and their relative leakiness and strength:

Identifier Sequence Induced Strength Leakyness Inducibilitya
Col. 2 CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA 14.2 0.203 69.9
Col. 3 CTGACGTAATCATACCGCCGAAAGTATTATCATTA 16.6 0.426 39
Col. 4 CTGACGCGACAACATTGCGTCCTATAAAATGCCGA 14.2 0.345 41
Col. 5 CTGACGGCCCGCCCCCGATGCGCATAAAATACCAA 17.7 0.424 41.6
Col. 12 CTGACGCGTATCGCGAGCGGGCGTTATTATACGCA 13.6 0.414 32.9
Col. 10 CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA 18 0.462 38.8
Col. 9 CTGACGCCACCCCCCCCCCCGGCGTATAATTCCCA 16.1 0.530 30.4
Col. 8 CTGACGCCCTACCGCTCGCCCCCCTATTATACCCA 13.4 0.211 63.4
Col. 13 CTGACGCCCCCCCCGTCCACCCCCTAATATCCCGA 10.9 0.096 113.6
Col. 15 CTGACGTGCGCCTGCCGTCCAAAGTAATATCCTTA 15.5 0.235 65.8
Col. 18 CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA 19.8 0.524 37.8
Col. 19 CTGACGCTCGCACCGGCGACCCGATAATATCCATA 18.0 0.315 57
Col. 31 CTGACGCCCCCCCTCCCCCGACCTAAAATATCCAG 3.8 0.161 23.6
Col. 29 CTGACGCCGCTGCACCCGTCCCCCTATTATAGCAA 14.8 0.301 49.2
Col. 26 CTGACGCCGATCCTATCTCCCTATTAAAATTTTTA 13.8 0.292 47.2
Col. 22 CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA 16.8 0.466 36.1
Col. 33 CTGACGCCAGCTTCCTACTTCAAATATAATCCGTA 14.4 1.01 14.3
Col. 34 CTGACGTCCCTCTTCCGCGCCCCCTAAAATACCCA 12.4 0.187 65.9
Col. 35 CTGACGCCCAACCCGACACAGCGATATAATAATGA 18.4 0.89 20.7