Difference between revisions of "Part:BBa K1028001"

Revision as of 12:53, 4 October 2013

INP.Si4 Silica Binding Complex

Introduction

This biobrick was designed utilise Ice Nucleation Protein to express the Si4 Binding Peptide on the extracellular surface of the plasma membrane of E.coli cells.

Fig.1 Illustrated schematic of the Si4 Binding Peptide binding presented on the C-terminus of INP as it bind with a silica microsphere.

Usage and Experimental Data

Fig.1: Monitoring the growth rate of cells containing Si4 Binding Peptide attached to the C-terminus of INP, labelled here as Device 2, within the PSB1C3 standard iGEM submission plasmid. Although the graph shows a log and a lag phase for the cells containing the insert plasmid, the absorbance reached in log phase is very low compared to the control cells in fact showing the poorest growth of any of the Leeds 2013 submitted parts.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 409
1000
COMPATIBLE WITH RFC[1000]

@@ Line 15: / Line 15: @@
 <img src="http://i276.photobucket.com/albums/kk29/JosephBeetBeet/INPSi4Growthcurve.png" style="width:400px;margin-right:160px"  ></a>
-<p style="text-align:center"><b>Fig.1:</b> Monitoring the growth rate of cells containing Si4 Binding Peptide attached to the C-terminus of INP, labelled here as Device 2, within the PSB1C3 standard iGEM submission plasmid. Although the graph shows a log and a lag phase for the cells containing the insert plasmid, the absorbance reached in log phase is very low compared to the control cells.
+<p style="text-align:center"><b>Fig.1:</b> Monitoring the growth rate of cells containing Si4 Binding Peptide attached to the C-terminus of INP, labelled here as Device 2, within the PSB1C3 standard iGEM submission plasmid. Although the graph shows a log and a lag phase for the cells containing the insert plasmid, the absorbance reached in log phase is very low compared to the control cells in fact showing the poorest growth of any of the Leeds 2013 submitted parts.
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