Difference between revisions of "Part:BBa K1065302:Design"
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The part consists of: <BR> | The part consists of: <BR> | ||
| − | <partinfo> | + | <partinfo>Bba_K1065305</partinfo>: the entire blue light sensing device with fluorescent output.<BR> |
<partinfo>BBa_R0010</partinfo>: pLac promoter (LacI regulated). | <partinfo>BBa_R0010</partinfo>: pLac promoter (LacI regulated). | ||
RBS sequence | RBS sequence | ||
Revision as of 17:13, 4 October 2013
Blue light sensing device without inverter for the production of amilGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 770
Illegal NgoMIV site found at 842
Illegal NgoMIV site found at 932
Illegal NgoMIV site found at 950
Illegal NgoMIV site found at 1462
Illegal NgoMIV site found at 1755
Illegal NgoMIV site found at 1849
Illegal AgeI site found at 484
Illegal AgeI site found at 1630 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1519
Illegal BsaI.rc site found at 383
Design Notes
Part BBa_K952003 was missing an RBS before the amilGFP CDS. We inserted the RBS by mutagenesis, and created the part BBa_K1065305. Then we added a pLAC promoter (R0010) in front of the device and characterized it.
Source
The part consists of:
BBa_K1065305: the entire blue light sensing device with fluorescent output.
BBa_R0010: pLac promoter (LacI regulated).
RBS sequence
References
- Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.
- Moglich A, Ayers RA and Moffat K. (2009) Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Bio. 385, 5, 1433-1444.
- Ohlendorf, R., Vidavski, R.R., Eldar, A., Moffat, K. & Möglich, A.(2012). From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. J. Mol. Biol., 416: 534: 542