Difference between revisions of "Part:BBa K1088003"
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This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The sequence have been optimized for E. coli codons and is made from the cDNA sequence "HRT2" produced by Kasem Asawatreratanakul et al. (1)
 
This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The sequence have been optimized for E. coli codons and is made from the cDNA sequence "HRT2" produced by Kasem Asawatreratanakul et al. (1)
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The rubber producing capabilities of HRT2 was assayed by the SDU-Denmark 2013 team by purifying the rubber content of bacteria expressing the HRT2 gene under control of arabinose promoter. The purification was done according to the SOP developed by the SDU-Denmark 2013 team and can be found at their wiki or by clicking here:[[https://static.igem.org/mediawiki/2013/4/48/IGEM2013_SOP0030_v01.pdf]]
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The purified rubber was investigated by H-NMR and the results indicated the prescense of rubber production. However, due to teqhnical difficulties, the experiments must be done again to ensure scientific certainty.
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[[File:SDU2013_Characterization_NMR_1.png]]
  
 
Reference: see design source
 
Reference: see design source

Revision as of 15:51, 4 October 2013

Cis-1,4-prenyltransferase obtained from Hevea brasiliensis cDNA

This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The sequence have been optimized for E. coli codons and is made from the cDNA sequence "HRT2" produced by Kasem Asawatreratanakul et al. (1)


The rubber producing capabilities of HRT2 was assayed by the SDU-Denmark 2013 team by purifying the rubber content of bacteria expressing the HRT2 gene under control of arabinose promoter. The purification was done according to the SOP developed by the SDU-Denmark 2013 team and can be found at their wiki or by clicking here:[[1]] The purified rubber was investigated by H-NMR and the results indicated the prescense of rubber production. However, due to teqhnical difficulties, the experiments must be done again to ensure scientific certainty.

File:SDU2013 Characterization NMR 1.png

Reference: see design source


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]