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Revision as of 23:20, 3 October 2013

NLS

dCas9
Function nuclear localization signal
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

The nuclear localization signal or nuclear localization sequence (NLS) is a specific amino acid sequence in nuclear proteins.
The NLS is recognized by importines, which enable the nuclear proteins the access to the nucleus.
It consists mostly of positively charged amino acids like lysine and arginine.

In our constructs the NLS found place in many proteins such as Cas9.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]