Difference between revisions of "Part:BBa K1033206"
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<partinfo><h1>Lactobacillus shuttlevector pSBLbC</h1>
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pSBLbC is a shuttle vector between E. coli, Lacotbacillus, and some other lactic acid bacteria. It has been used for subcloning in E. coli and to express chromoproteins amilCP and aeBlue. For use in Lactobacillus we recommend the version with erythromycin resistance (pSBLbC: [[part:Part:BBa_K1033207|]]) since it has been successfully used to transfer genes between the two species.
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<h1>Lactobacillus shuttlevector pSBLbC</h1>
  
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It was made by replacing the replicon of BioBrick compatible plasmid pSB4C15 ([[Part:BBa_K864001|]]) with a broad range replicon from the <i>Lactococcus lactis</i> engineered plasmid  pJP059. The replicon is also referred to as pSH71and and is related to pWV01 and that family of rolling circle replicating plasmids.</partinfo>
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pSBLbC is a shuttle vector between E. coli, Lacotbacillus, and some other lactic acid bacteria. It has been used for subcloning in E. coli and to express chromoproteins amilCP and aeBlue. For use in Lactobacillus we recommend the version with erythromycin resistance (pSBLbC: [[part:Part:BBa_K1033207|Part:BBa_K1033207]]) since it has been successfully used to transfer genes between the two species.
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It was made by replacing the replicon of BioBrick compatible plasmid pSB4C15 ([[Part:BBa_K864001|BBa_K864001]]) with a broad range replicon from the <i>Lactococcus lactis</i> engineered plasmid  pJP059. The replicon is also referred to as pSH71and and is related to pWV01 and that family of rolling circle replicating plasmids.
  
 
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Revision as of 20:36, 3 October 2013

No argument specified with partinfo tag.

Lactobacillus shuttlevector pSBLbC

pSBLbC is a shuttle vector between E. coli, Lacotbacillus, and some other lactic acid bacteria. It has been used for subcloning in E. coli and to express chromoproteins amilCP and aeBlue. For use in Lactobacillus we recommend the version with erythromycin resistance (pSBLbC: Part:BBa_K1033207) since it has been successfully used to transfer genes between the two species.

It was made by replacing the replicon of BioBrick compatible plasmid pSB4C15 (BBa_K864001) with a broad range replicon from the Lactococcus lactis engineered plasmid pJP059. The replicon is also referred to as pSH71and and is related to pWV01 and that family of rolling circle replicating plasmids.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3036
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3036
    Illegal NheI site found at 1981
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3036
    Illegal BamHI site found at 1960
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3036
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3036
    Illegal XbaI site found at 3051
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]