Difference between revisions of "Part:BBa K1065106"

Revision as of 20:20, 3 October 2013

Wintergreen device with SAM: Plac + PCHBA + araCpBAD+ BSMT1 + Plac + SAM synthetase

This is the complete wintergreen odor device from iGEM UniTN-Trento 2013 which encodes for four enzymes: PchB, PchA, BSMT1 and SAM. The device produces methyl salicylate (a fruit ripening inhibitor) and was used for the development of the project B. Fruity by UniTN Trento 2013. This part, containing SAM enzyme BBa_K1065105, is an improvement of part BBa_K1065102.

Usage and Biology

The chorismate, a metabolic intermediate of the Shikimate pathway which many plants and bacteria have, undergoes firstly a reaction of isomerization by the isochorismate synthase, PchA . Salicylate is then obtained by the action of PchB an isochorismate pyruvate lyase. Both enzymes are from the micro-organism Pseudomonas aeruginosa . In the final part of the reaction, BSMT1, a methyltransferase, transfers a methyl group from the S-adenosyl-L-methionine synthesized by the SAM synthetase.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 3107
Illegal BamHI site found at 3856
Illegal BamHI site found at 4736
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 389
Illegal NgoMIV site found at 793
Illegal NgoMIV site found at 918
Illegal NgoMIV site found at 929
Illegal NgoMIV site found at 1210
Illegal NgoMIV site found at 1678
Illegal AgeI site found at 2942
Illegal AgeI site found at 5316
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3397
Illegal SapI site found at 2924

@@ Line 8: / Line 8: @@
 ===Usage and Biology===
-The chorismate, a metabolic intermediate of the Shikimate pathway which many plants and bacteria (like E.coli and B.subtilis ) have, undergoes firstly a reaction of isomerization by the isochorismate synthase, PchA .  Salicylate is then obtained by the action of PchB an isochorismate pyruvate lyase. Both enzymes are from the micro-organism Pseudomonas aeruginosa . In the final part of the reaction, BSMT1, a methyltransferase, transfers a methyl group from the S-adenosyl-L-methionine synthesized by the SAM synthetase.
+The chorismate, a metabolic intermediate of the Shikimate pathway which many plants and bacteria have, undergoes firstly a reaction of isomerization by the isochorismate synthase, PchA .  Salicylate is then obtained by the action of PchB an isochorismate pyruvate lyase. Both enzymes are from the micro-organism Pseudomonas aeruginosa . In the final part of the reaction, BSMT1, a methyltransferase, transfers a methyl group from the S-adenosyl-L-methionine synthesized by the SAM synthetase.
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