Difference between revisions of "Part:BBa K1033206"
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− | <partinfo> | + | <partinfo><h1>Lactobacillus shuttlevector pSBLbC</h1> |
− | + | pSBLbC is a shuttle vector between E. coli, Lacotbacillus, and some other lactic acid bacteria. It has been used for subcloning in E. coli and to express chromoproteins amilCP and aeBlue. For use in Lactobacillus we recommend the version with erythromycin resistance (pSBLbC: [[part:Part:BBa_K1033207|]]) since it has been successfully used to transfer genes between the two species. | |
+ | |||
+ | It was made by replacing the replicon of BioBrick compatible plasmid pSB4C15 ([[Part:BBa_K864001|]]) with a broad range replicon from the <i>Lactococcus lactis</i> engineered plasmid pJP059. The replicon is also referred to as pSH71and and is related to pWV01 and that family of rolling circle replicating plasmids.</partinfo> | ||
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Revision as of 20:35, 3 October 2013
No part name specified with partinfo tag.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 3036
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3036
Illegal NheI site found at 1981
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3042 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3036
Illegal BamHI site found at 1960 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 3036
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 3036
Illegal XbaI site found at 3051
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000COMPATIBLE WITH RFC[1000]