Difference between revisions of "Part:BBa K1075020"

Revision as of 20:46, 3 October 2013

E. coli ssrA(DAS+4)

construction

The part contains the mutated E. coli(ec)ssrA(DAS+4) tag.

biology

The ecssrA tag is a short peptide sequence, which is fused to the C-terminus of proteins, which should be degraded. It relates to the protease ClpXP complex in E.coli and it also allows the adaptor protein ecSspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutated ecssrA (DAS+4) weakens a direct binding between proteases and ecssrA(DAS+4) and increases the dependance of ecSspB. [http://www.ncbi.nlm.nih.gov/pubmed/16762842]

application

The ecssrA(DAS+4) tag can be fused to proteins, so they get degraded. The tag can be used by everyone e.g. to research the function of a protein.

Sequence and Features