Difference between revisions of "Part:BBa K1033223"

Revision as of 17:21, 4 October 2013

Promoter CP30

CP30 is a promoter in the CP collection with the strength of 0.50 compared to the promoter J23101 in standard parts (J23101 was used as reference and its strength were put to 1).

Above you can see a diagram of all the CP promoters strength relative to J23101 [RPU]. The CP promoter parts is a collection of constitutive promoters characterized in both E. coli and Lactococcus lactis by Jensen and Hammer (1998). In E. coli it has also been characterized by us in a fluorescence-activated cell sorting machine (FACS) with BFP using the constitutive promoter J23101 in the standard parts as a reference.

In the article by Jensen and Hammer they stated that these promoters were based on the consensus sequence of promoters in gram-positive bacterium L. lactis, though there are no obvious reasons why they shouldn’t work in other organisms. Jensen and Hammer characterized them in both L. lactis and gram-negative bacterium E. coli using the reporter gene lacLM. This indicates that there is a possibility that these promoters are near universally applicable to prokaryotic organisms in general.

Sequence and Features