Difference between revisions of "Part:BBa K1065101"
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<partinfo>BBa_K1065101 short</partinfo> | <partinfo>BBa_K1065101 short</partinfo> | ||
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The part <partinfo>BBa_K1065100</partinfo> is under the control of the inducible promoter AraC-pBAD. This promoter allows to produce methyl salicylate starting from salicylic acid when 5 mM of Arabinose is added | The part <partinfo>BBa_K1065100</partinfo> is under the control of the inducible promoter AraC-pBAD. This promoter allows to produce methyl salicylate starting from salicylic acid when 5 mM of Arabinose is added | ||
to the culture. | to the culture. | ||
Revision as of 14:57, 3 October 2013
AraCpBAD + BSMT1
The part BBa_K1065100 is under the control of the inducible promoter AraC-pBAD. This promoter allows to produce methyl salicylate starting from salicylic acid when 5 mM of Arabinose is added to the culture.
This part was cloned by the iGEM UniTN Trento 2013 team for the construction of Wintergreen Device BBa_K1065106.
Usage and Biology
BSMT1 catalyzes the conversion of salicylic acid to methyl salicylate, which has a wintergreen smell.
The arabinose inducible promoter BBa_K731201 was fully characterized by iGEM UniTN Trento 2012.
The arabinose inducible promoter BBa_K731201 was fully characterized by iGEM UniTN Trento 2012.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 1893 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1434
Illegal SapI site found at 961