Difference between revisions of "Part:BBa K1065106"

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This is the complete wintergreen odor device from iGEM UniTN-Trento 2013 which encodes for four enzymes: PchB, PchA, BSMT1 and SAM.
 
This is the complete wintergreen odor device from iGEM UniTN-Trento 2013 which encodes for four enzymes: PchB, PchA, BSMT1 and SAM.
 
The device produces methyl salicylate (a fruit ripening inhibitor) and was used for the development of the project B. Fruity by UniTN Trento 2013.  
 
The device produces methyl salicylate (a fruit ripening inhibitor) and was used for the development of the project B. Fruity by UniTN Trento 2013.  
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This part, containing SAM enzyme <partinfo>BBa_K1065105</partinfo>, is an improvement of part <infopart>BBa_K1065102</partinfo>.  
+
This part, containing SAM enzyme <partinfo>BBa_K1065105</partinfo>, is an improvement of part <partinfo>BBa_K1065102</partinfo>.  
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 14:51, 3 October 2013

Wintergreen device with SAM: Plac + PCHBA + araCpBAD+ BSMT1 + Plac + SAM synthetase

This is the complete wintergreen odor device from iGEM UniTN-Trento 2013 which encodes for four enzymes: PchB, PchA, BSMT1 and SAM. The device produces methyl salicylate (a fruit ripening inhibitor) and was used for the development of the project B. Fruity by UniTN Trento 2013. This part, containing SAM enzyme BBa_K1065105, is an improvement of part BBa_K1065102.

Usage and Biology

The chorismate, a metabolic intermediate of the Shikimate pathway which many plants and bacteria (like E.coli and B.subtilis ) have, undergoes firstly a reaction of isomerization by the isochorismate synthase, PchA and then the salicylate is obtained by the action of PchB an isochorismate pyruvate lyase. Both enzymes are from the micro-organism Pseudomonas aeruginosa . In the final part of the reaction, BSMT1, a methyltransferase, transfers a methyl group from the S-adenosyl-L-methionine synthesized by the SAM synthetase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3107
    Illegal BamHI site found at 3856
    Illegal BamHI site found at 4736
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 793
    Illegal NgoMIV site found at 918
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1210
    Illegal NgoMIV site found at 1678
    Illegal AgeI site found at 2942
    Illegal AgeI site found at 5316
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3397
    Illegal SapI site found at 2924