Difference between revisions of "Part:BBa K1122674"

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<span class='h3bb'>Activity of adhB as a fusion member</span>
 
<span class='h3bb'>Activity of adhB as a fusion member</span>
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<partinfo>BBa_K1122674 Activity of adhB as a fusion member</partinfo>
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[[File:bioethanol8.jpg]]
 
[[File:bioethanol8.jpg]]
  
 
<span class='h3bb'>Microscopy of cells expressing fused protein</span>
 
<span class='h3bb'>Microscopy of cells expressing fused protein</span>
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<partinfo>BBa_K1122674 Microscopy of cells expressing fused protein</partinfo>
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[[File:bioethanol9.jpg]]
 
[[File:bioethanol9.jpg]]

Revision as of 13:46, 3 October 2013

Plac+fused PDC-ADH

lac promoter plus PDC-ADH fusion protein for ethanol production


Generation of protein fusion

Bioethanol1.jpg

Bioethanol2.jpg

Evidence for presence of protein fusion

DNA level evidence


Bioethanol3.jpg

File:Bioethanol sequencing.jpg


Protein level evidence


Bioethanol4.jpg

Bioethanol5.jpg

Bioethanol6.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1109
    Illegal AgeI site found at 2315
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

No part name specified with partinfo tag.

Bioethanol7.jpg

Activity of adhB as a fusion member


Bioethanol8.jpg

Microscopy of cells expressing fused protein


Bioethanol9.jpg