Difference between revisions of "Part:BBa K1122674"

(Generation of protein fusion)
(Evidence for presence of protein fusion)
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<partinfo>BBa_K1122674 DNA level evidence</partinfo>
 
<partinfo>BBa_K1122674 DNA level evidence</partinfo>
  
[[File:bioethanol3]]
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[[File:bioethanol3.jpg]]
  
[[File:bioethanol_sequencing]]
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[[File:bioethanol_sequencing.jpg]]
  
  
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<partinfo>BBa_K1122674 Protein level evidence</partinfo>
 
<partinfo>BBa_K1122674 Protein level evidence</partinfo>
  
[[File:bioethanol4]]
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[[File:bioethanol4.jpg]]
  
[[File:bioethanol5]]
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[[File:bioethanol5.jpg]]
  
[[File:bioethanol6]]
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[[File:bioethanol6.jpg]]
  
  
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<partinfo>BBa_K1122674 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1122674 SequenceAndFeatures</partinfo>
  
 
 
 
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>Ethanol production using fused pdc and adhB</partinfo>
 
<partinfo>Ethanol production using fused pdc and adhB</partinfo>

Revision as of 13:37, 3 October 2013

Plac+fused PDC-ADH

lac promoter plus PDC-ADH fusion protein for ethanol production


Generation of protein fusion

Bioethanol1.jpg

Bioethanol2.jpg

Evidence for presence of protein fusion

DNA level evidence BBa_K1122674 DNA level evidence Not understood

Bioethanol3.jpg

File:Bioethanol sequencing.jpg


Protein level evidence BBa_K1122674 Protein level evidence Not understood

Bioethanol4.jpg

Bioethanol5.jpg

Bioethanol6.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1109
    Illegal AgeI site found at 2315
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

No part name specified with partinfo tag.

File:Bioethanol7

Activity of adhB as a fusion member BBa_K1122674 Activity of adhB as a fusion member Not understood

File:Bioethanol8

Microscopy of cells expressing fused protein BBa_K1122674 Microscopy of cells expressing fused protein Not understood

File:Bioethanol9