Difference between revisions of "Part:BBa K1122674"

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(→‎Usage and Biology)
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===Usage and Biology===
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===Generation of protein fusion===
  
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[[File:bioethanol1]]
  
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[[File:bioethanol2]]
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===Evidence for presence of protein fusion===
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<span class='h3bb'>DNA level evidence</span>
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<partinfo>BBa_K1122674 DNA level evidence</partinfo>
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[[File:bioethanol3]]
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[[File:bioethanol_sequencing]]
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<span class='h3bb'>Protein level evidence</span>
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<partinfo>BBa_K1122674 Protein level evidence</partinfo>
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[[File:bioethanol4]]
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[[File:bioethanol5]]
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[[File:bioethanol6]]
  
  
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1122674 parameters</partinfo>
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<partinfo>Ethanol production using fused pdc and adhB</partinfo>
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<!-- -->
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[[File:bioethanol7]]
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<span class='h3bb'>Activity of adhB as a fusion member</span>
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<partinfo>BBa_K1122674 Activity of adhB as a fusion member</partinfo>
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[[File:bioethanol8]]
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<span class='h3bb'>Microscopy of cells expressing fused protein</span>
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<partinfo>BBa_K1122674 Microscopy of cells expressing fused protein</partinfo>
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[[File:bioethanol9]]

Revision as of 13:28, 3 October 2013

Plac+fused PDC-ADH

lac promoter plus PDC-ADH fusion protein for ethanol production


Generation of protein fusion

File:Bioethanol1

File:Bioethanol2


Evidence for presence of protein fusion

DNA level evidence BBa_K1122674 DNA level evidence Not understood

File:Bioethanol3

File:Bioethanol sequencing


Protein level evidence BBa_K1122674 Protein level evidence Not understood

File:Bioethanol4

File:Bioethanol5

File:Bioethanol6


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1109
    Illegal AgeI site found at 2315
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters

No part name specified with partinfo tag.

File:Bioethanol7

Activity of adhB as a fusion member BBa_K1122674 Activity of adhB as a fusion member Not understood

File:Bioethanol8

Microscopy of cells expressing fused protein BBa_K1122674 Microscopy of cells expressing fused protein Not understood

File:Bioethanol9