Difference between revisions of "Part:BBa C0062:Experience"

Revision as of 00:06, 4 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_C0062

User Reviews

UNIQ0d6f16b50862df8e-partinfo-00000000-QINU

•••••

SUN(Tsinghua)

Part was sequenced and functional. LuxR was used in our Portable Pathogen Detector.

;

•••••

wmholtz

Using this part, I have successfully constructed and tested a quorum sensing circuit in E. coli.

;

•••••

Youri

This part was used and tested as a subpart in K546000, K546001, K546002, K546003, K546005 and K546546. This part functioned in all cases.

;

Kevin (iGEM Braunschweig 2013)

The plasmid pSB1C3 BBa_C0062 from the 2013 distribution Kit was transformed in E. coli XL1 BlueMRF. Sequencing with standard verification primers VR and VF2 confirmed matching sequence of backbone DNA up to the EcoRI restriction site. The rest of the sequence does not match the registry entry. A restriction assay (Figure 1) showed that the sequenced part has no XbaI restriction site following the EcoRI site indicating another part in front of BBa_C0062 with a length of at least 1000 bp.

;

UNIQ0d6f16b50862df8e-partinfo-00000005-QINU

@@ Line 47: / Line 47: @@
 <I>Kevin (iGEM Braunschweig 2013)</I>
 |width='60%' valign='top'|
-The plasmid pSB1C3 BBa_C0062 from the 2013 distribution Kit was transformed in <I>E. coli</I> XL1 BlueMRF. Sequencing with standard verification primers VR and VF2 confirmed matching sequence of backbone DNA up to the EcoRI restriction site. The rest of the sequence does not match the registry entry. A restriction assay showed that the sequenced part has no XbaI restriction site following the EcoRI site indicating another part in front of BBa_C0062 with a length of at least 1000 bp.
+The plasmid pSB1C3 BBa_C0062 from the 2013 distribution Kit was transformed in <I>E. coli</I> XL1 BlueMRF. Sequencing with standard verification primers VR and VF2 confirmed matching sequence of backbone DNA up to the EcoRI restriction site. The rest of the sequence does not match the registry entry. A restriction assay (Figure 1) showed that the sequenced part has no XbaI restriction site following the EcoRI site indicating another part in front of BBa_C0062 with a length of at least 1000 bp.
+[[File:iGEM62Testverdau.png| 300px | Figure 1: restriction assay of BBa_C0062 with the indicated restriction enzymes]]
 |};
 <!-- DON'T DELETE --><partinfo>BBa_C0062 EndReviews</partinfo>