Difference between revisions of "Part:BBa K1111014"
Sandelisa90 (Talk | contribs) (→Usage and Biology) |
Sandelisa90 (Talk | contribs) (→Usage and Biology) |
||
| Line 12: | Line 12: | ||
<br>Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria. | <br>Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria. | ||
| − | |||
==<span class='h3bb'>Sequence and Features</span>== | ==<span class='h3bb'>Sequence and Features</span>== | ||
<partinfo>BBa_K1111014 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1111014 SequenceAndFeatures</partinfo> | ||
Revision as of 21:23, 2 October 2013
Ice Nucleation Protein fused to Streptavidin BBa_K283010
Introduction
It is a fusion protein between an Ice Nucleation Protein (INP) and Streptavidin Alive made by Gibson Assembly. INP is an outer membrane protein foud in the genome of Pseudomonas syringae, that is recognize by the E.coli protein secretion machinery.
Usage and Biology
This part was designed to express streptavidin on the outer membrane of E.coli in order to attach biotinylated nanoparticles to the cells.
Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1649
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1036
Illegal AgeI site found at 1691
Illegal AgeI site found at 1742 - 1000COMPATIBLE WITH RFC[1000]