Difference between revisions of "Part:BBa K1111014"
(→‎Usage and Biology)
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==Usage and Biology==
 
==Usage and Biology==
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For us, the idea was to attach nanoparticles covered with biotin, using the strong affinity between both, but any experience implying biotin can be considered.
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This part was designed to express streptavidin on the outer membrane of E.coli in order to attach biotinylated nanoparticles to the cells. 
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<br>Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.
  
 
Thanks to the  
 
Thanks to the  
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<span class='h3bb'>Sequence and Features</span>
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==<span class='h3bb'>Sequence and Features</span>==
 
<partinfo>BBa_K1111014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1111014 SequenceAndFeatures</partinfo>
  

Revision as of 21:22, 2 October 2013

Ice Nucleation Protein fused to Streptavidin BBa_K283010

Introduction

It is a fusion protein between an Ice Nucleation Protein (INP) and Streptavidin Alive made by Gibson Assembly. INP is an outer membrane protein foud in the genome of Pseudomonas syringae, that is recognize by the E.coli protein secretion machinery.

Usage and Biology

This part was designed to express streptavidin on the outer membrane of E.coli in order to attach biotinylated nanoparticles to the cells.
Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.

Thanks to the

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1649
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 1691
    Illegal AgeI site found at 1742
  • 1000
    COMPATIBLE WITH RFC[1000]