Difference between revisions of "Part:BBa K1151002:Design"
LeleBiotec (Talk | contribs) (→Design Notes) |
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'''Figure 1:''' PCR results of the intergenic region. | '''Figure 1:''' PCR results of the intergenic region. | ||
− | Primers used (including Biobricks' Prefix and Suffix, lowercase): | + | |
+ | '''Primers used''' (including Biobricks' Prefix and Suffix, lowercase): | ||
''IntergenicaFor:'' gtttcttcgaattcgcggccgcttctagagTGAGAAAAATCCTTTTTTG | ''IntergenicaFor:'' gtttcttcgaattcgcggccgcttctagagTGAGAAAAATCCTTTTTTG |
Revision as of 19:07, 2 October 2013
Double divergent promoter pnikR-pexbB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was amplified by PCR from the plasmid received.
Figure 1: PCR results of the intergenic region.
Primers used (including Biobricks' Prefix and Suffix, lowercase):
IntergenicaFor: gtttcttcgaattcgcggccgcttctagagTGAGAAAAATCCTTTTTTG
pexbrev: gtttcttcctgcagcggccgctactagtaGCACCCTATAAGAAGGCATC
Source
This part comes from H. pylori genomic sequence and it was amplified by PCR.
References
1. Delany et al, In vitro analysis of protein-operator interactions of the NikR and Fur Metal-Responsive Regulators of coregulated genes in Helicobacter pylori. J Bacteriol. 2005 Nov;187(22):7703-15.