Difference between revisions of "Part:BBa K1111012"

(→‎Introduction)
(→‎Usage and Biology)
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This part was designed to express streptavidin on the outer membrane of E.coli in order to attach biotinylated nanoparticles to the cells.   
 
This part was designed to express streptavidin on the outer membrane of E.coli in order to attach biotinylated nanoparticles to the cells.   
 
<br>Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.
 
<br>Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.
 +
<br>For further Characterization, refer to the datasheet below.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 18:24, 2 October 2013

Ice Nucleation Protein fused to Streptavidin Alive

Introduction

It is a fusion protein between an Ice Nucleation Protein (INP) and Streptavidin Alive made by Gibson Assembly. INP is an outer membrane protein foud in the genome of Pseudomonas syringae, that is recognize by the E.coli protein secretion machinery.

Usage and Biology

This part was designed to express streptavidin on the outer membrane of E.coli in order to attach biotinylated nanoparticles to the cells.
Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.
For further Characterization, refer to the datasheet below.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1727
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1649
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1727
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1727
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 1691
    Illegal AgeI site found at 1742
  • 1000
    COMPATIBLE WITH RFC[1000]