Difference between revisions of "Part:BBa K1111012"
Sandelisa90 (Talk | contribs) (→what it is) |
Sandelisa90 (Talk | contribs) (→Introduction) |
||
| Line 3: | Line 3: | ||
==Introduction== | ==Introduction== | ||
| − | + | It is a fusion protein between an Ice Nucleation Protein (INP) and Streptavidin Alive made by Gibson Assembly. | |
INP is an outer membrane protein foud in the genome of Pseudomonas syringae, that is recognize by the E.coli protein secretion machinery. | INP is an outer membrane protein foud in the genome of Pseudomonas syringae, that is recognize by the E.coli protein secretion machinery. | ||
This Biobrick is under the control of a Lac promoter. | This Biobrick is under the control of a Lac promoter. | ||
Revision as of 18:21, 2 October 2013
Ice Nucleation Protein fused to Streptavidin Alive
Introduction
It is a fusion protein between an Ice Nucleation Protein (INP) and Streptavidin Alive made by Gibson Assembly. INP is an outer membrane protein foud in the genome of Pseudomonas syringae, that is recognize by the E.coli protein secretion machinery. This Biobrick is under the control of a Lac promoter. For further Characterization, refer to the datasheet below.
Usage and Biology
This part was designed to express streptavidin on the outer membrane of E.coli in order to attach biotinylated nanoparticles to the cells.
Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1727
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1649
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1727
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1727
Illegal NgoMIV site found at 1036
Illegal AgeI site found at 1691
Illegal AgeI site found at 1742 - 1000COMPATIBLE WITH RFC[1000]