Difference between revisions of "Part:BBa K1111012"

Revision as of 17:59, 2 October 2013

Ice Nucleation Protein fused to Streptavidin Alive

Introduction

What it is : INP sequence fused with Streptavidin Alive by gibson assembly. The INP-streptavidin construct expresses streptavidin at the outer membrane of E.Coli. INP is an outer membrane protein foud in the genome of Pseudomonas syringae. It is under the control of Lac promoter and thus is constitutively expressed in E.Coli. The expression of this plasmid was tested by transforming cells and looking for microscopy at either of a fluorescent antibody again streptavidin or a fluorescent biotin (biotin and streptavidin have a strong affinity, their Kd is 10-15 M).

Usage and Biology

This part was designed to express streptavidin on the outer membrane of E.coli in order to attach biotinylated nanoparticles to the cells.
Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1727
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1649
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1727
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1727
Illegal NgoMIV site found at 1036
Illegal AgeI site found at 1691
Illegal AgeI site found at 1742
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 This part was designed to express streptavidin on the outer membrane of E.coli in order to attach biotinylated nanoparticles to the cells.
-Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.
+<br>Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.
 <span class='h3bb'>Sequence and Features</span>