Difference between revisions of "Part:BBa J37027:Design"

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(→‎Design Notes)
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This part contains multiple reading frames so cannot be correctly represented in this version of the registary.
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'''This part contains multiple reading frames so cannot be correctly represented in this version of the registary.'''
  
 
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The sequence is correct however the annotations are not. Anything said in this summary trumps the registary site
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The sequence is correct however the annotations are not. Anything said in [https://static.igem.org/mediawiki/parts/e/e0/Cre_PCR3.pdf '''this summary'''] trumps the registary site.
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John
  
 
===Source===
 
===Source===

Revision as of 09:46, 2 August 2006

Pops Blocker (Cre/Lox system)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 340
    Illegal AgeI site found at 452
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains multiple reading frames so cannot be correctly represented in this version of the registary.

The sequence is correct however the annotations are not. Anything said in this summary trumps the registary site.

John

Source

From Registry: BBa_B0015 BBa_I13521

Lox Sites: Zuwen Zhang and Beat Lutz (2002) Cre Recombinase-mediated inversion using lox66 and lox71: method to introduce conditional point mutations into the CREB-binding protein NUCLEAIC ACIDS RESEARCH

References